[Evaluation of relationship between serum cholesterol and triglyceride level and gallstone incidence]

Cholecystectomy is one of the most common operations in the field of general surgery. It has higher cost in operations with complication and in these cases mortality and morbidity will increase. The main goal of this research is the study and compare of cholesterol and triglyceride serum level in gallstone group and control group. The role of Serum total cholesterol and triglyceride level in the etiology of gallstone disease was assessed in a case control study in Emam Reza hospital. Study include 37 cases [15 males and 22 females] with surgically or ultrasonographicaly confirmed cholecystolithiasis and 35 cases [16 males and 19 females] as control outpatient and hospital admitted patient without debilitating disease [such as umbilical and inguinal Hernia] over age of forty. Mean age in stone group was 54 years and in control group was 57 years. Serum cholesterol and triglyceride concentration in gallstone patient was 193 mg/dl and 135.9 mg/dl respectively were compared with control series with cholesterol and triglyceride levels 176 mg/dl and 121.8 mg/dl respectively [p=0.258 for cholesterol and p=0.368 for triglyceride]. Gallstone patient had higher serum cholesterol and triglyceride level than control group but these differences were not statistically significant

Immunogenicity of entamoeba histolytica crude and fractionated antigens in animal model

Introduction: The immunogenicity of crude amoebic antigen and its fractions preparedfrom Entamoeba histolytica [NIH:200] was evaluated in experimental animals

Material and Methods: Forty two guinea pigs of either sex free from Entamoebainfection and aged around 3 to 4 weeks were randomly divided into 5 groups. The treatedgroups consisted of 8, 10, 6, and 8 animals and 10 animals served as controls. Crude amoebicextract and its chromatographed fractions were used to immunize the treated animals. All theanimals were assessed for immunity status, challenged with Entamoeba trophozoites andsubsequently examined for lesions of the caecum and liver

Results: Of the 8 animals immunized with crude antigen, one had liver abscess and 5 hadcaecal lesions. None of the 10 animals immunized with fraction I [FI] had hepatic lesions and one had caecal lesions. Both caecal and hepatic lesions were observed in animals immunizedwith FII and FIII

Conclusion: Results show that vaccination with the F1 fraction of Entamoeba histolyticaprovided up to 90% protection against infection. The other fractions and the crude extractprovided less protection